推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
BC011, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... CTSD(1509)
一般說明
Anti-Cathepsin D (Ab-1), mouse monoclonal, clone BC011, recognizes latent and mature form and the ~34 kDa subunit in breast carcinoma tissue extracts. It is validated for WB and for paraffin sections.
Purified mouse monoclonal antibody generated by immunizing CB6F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~52 kDa pro-cathespin D, the ~48 kDa mature cathepsin D, and the ~34 kDa subunit of cathespin D.
Recognizes the ~52 kDa latent form, the ~48 kDa mature form, and the ~34 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
免疫原
mature, human cathepsin D
應用
Immunoblotting (1 µg/ml)
Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
其他說明
Does not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.
Rochefort, H. 1992. Eur. J. Cancer.28A, 1780.
Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell20, 352.
Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.
Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell20, 352.
Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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