HIGFMAG-52K
MILLIPLEX® Human IGF-I, II Magnetic Bead Panel - Endocrine Assay
The Human IGF Panel, using the Luminex xMAP technology, enables the simultaneous analysis of one or both IGF-I and IGF-II protein biomarkers in human serum, plasma and cell/tissue culture samples.
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About This Item
分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84
推荐产品
品質等級
物種活性
human
製造商／商標名
Milliplex®
assay range
accuracy: 89%
(IGF-II)
sensitivity: 19 pg/mL
(MinDC+2SD; IGF-I)
sensitivity: 919 pg/mL
(MinDC+2SD; IGF-II)
standard curve range: 41-30,000 pg/mL
(IGF-I)
standard curve range: 892-650,000 pg/mL
(IGF-II)
inter-assay cv: <15%
intra-assay cv: <10%
(IGF-I)
inter-assay cv: <15%
intra-assay cv: <10%
(IGF-II)
技術
multiplexing: suitable
UniProt登錄號
檢測方法
fluorometric (Luminex xMAP)
運輸包裝
wet ice
基因資訊
human ... IGF1(3479)
一般說明
Insulin-like Growth Factors I and II are polypeptide, endocrine hormones similar in molecular structure to insulin. Both IGF-I and IGF-II are produced primarily by the liver and their bio-availabilities are regulated by the IGF binding proteins (IGFBPs).
MILLIPLEX® Human IGF-I, II Panel is a 2-plex kit to be used for the quantitative determination of IGF-I and/or IGF-II in human serum, plasma, tissue/cell extracts, or culture media samples. Reagents are included for the extraction of serum and plasma samples resulting in a 1:60 final dilution. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
MILLIPLEX® Human IGF-I, II Panel is a 2-plex kit to be used for the quantitative determination of IGF-I and/or IGF-II in human serum, plasma, tissue/cell extracts, or culture media samples. Reagents are included for the extraction of serum and plasma samples resulting in a 1:60 final dilution. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
特異性
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
應用
- Analytes: IGF-I, IGF-II
- Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: Use 25 μL per well of the final 1:60 extracted serum or plasma; Cell/tissue culture supernatants or lysates may require a dilution in appropriate control medium.
- NOTE: Sample extraction, using the reagents included in the kit, is required for serum and plasma samples. The final dilution for extracted serum or plasma samples is 1:60.
- Assay Run Time: Overnight (16-20 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Metabolic Disorders
特點和優勢
Design your multiplex kit by choosing available analytes within this panel.
包裝
Everything you need in a single kit.
96 well plate
儲存和穩定性
Recommended storage for kit components is 2 - 8°C.
其他說明
Please contact Technical Service for linearity of dilution.
Standard curve range: Refer to analysis sheet for exact standard concentracions.
法律資訊
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Sens. 1
儲存類別代碼
3 - Flammable liquids
Effects of maternal obstructive sleep apnoea on fetal growth: a prospective cohort study.
Fung, AM; Wilson, DL; Lappas, M; Howard, M; Barnes, M; O'Donoghue, F; Tong, S; Esdale et al.
Testing null
Kenneth Maleta et al.
The American journal of clinical nutrition, 113(2), 380-390 (2021-01-01)
Insulin-like growth factor I (IGF-I) is the most important hormonal promoter of linear growth in infants and young children. The objectives of this study were to compare plasma IGF-I concentration in a low- compared with a high-income country and characterize
Francesca D'Addio et al.
Nature communications, 13(1), 684-684 (2022-02-05)
Loss of pancreatic beta cells is a central feature of type 1 (T1D) and type 2 (T2D) diabetes, but a therapeutic strategy to preserve beta cell mass remains to be established. Here we show that the death receptor TMEM219 is
相关内容
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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