推荐产品
生物源
mouse
品質等級
共軛
PE
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
4G10, monoclonal
物種活性
vertebrates, human
製造商／商標名
Milli-Mark®
技術
flow cytometry: suitable
同型
IgG2bκ
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pTyr)
基因資訊
human ... PID1(55022)
一般說明
Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
特異性
Antibody recognizes tyrosine-phosphorylated proteins from all species
Expected to detect tyrosyne phosphorylated proteins in all species.
應用
Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.
品質
Evaluated by flow cytometry using EGF treated versus untreated A431 cells
標靶描述
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
外觀
Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
分析報告
Control
Treated versus untreated A431 cells
Treated versus untreated A431 cells
法律資訊
4G10 is a registered trademark of Upstate Group, Inc.
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Veronika Horkova et al.
Cell reports, 30(5), 1504-1514 (2020-02-06)
Overtly self-reactive T cells are removed during thymic selection. However, it has been recently established that T cell self-reactivity promotes protective immune responses. Apparently, the level of self-reactivity of mature T cells must be tightly balanced. Our mathematical model and experimental data
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