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形狀
liquid
包裝
pkg of 6 X 175 μg
技術
cell culture | stem cell: suitable
儲存溫度
2-8°C
應用
Xeno-free laminin-511 coating for feeder-free pluripotent stem cell cultures, 1050 μg (CHO-S derived)
成分
6 X 175 μg ECMatrix-511 E8 Laminin Substrate (0.5 mg/mL in PBS). Expressed in CHO-S cells.
品質
- Purity (SDS-Page): > 95%
- Endotoxin Test: = 750 EU/mg
- Mycoplasma Test: Negative
- Sterility Test: Negative
- Integrin Binding Assay (kDa): = 10 nM
準備報告
Depending on application, either a precoating or non-precoating method can be used to culture pluripotent stem cells.
Non-Precoating Method:
1. Detach cells into small clumps or single cells using Accutase.
2. Add ECMatrix-511 to fresh media at a final concentration of 0.25 μg/cm2 (for example: for one well of a 6-well plate add 5 uL of the 0.5 mg/mL stock solution).
3. Add cells to the ECMatrix-511/Media and plate the cells at desired density.
Precoating Method
1. Dilute the 0.5 mg/mL stock solution with sterile PBS to achieve a 2.5 ug/mL working solution.
2. Coat dishes with ECMatrix-511 at 0.25 μg/cm2 (for example, for one well of a 6-well plate add 1 mL of the 2.5 μg/mL working solution).
3. Incubate for 1 hour at 37°C, 3 hours at room temperate or overnight at 4°C.
4. Before use, remove remaining fluid from the coated surface (do not rinse).
5. Detach cells into small clumps using Accutase.
6. Plate the cells at desired density.
Note: Do not allow the plates to dry, briefly spin down all liquids in the tube before use, avoid repeated freeze-thaw cycles.
Non-Precoating Method:
1. Detach cells into small clumps or single cells using Accutase.
2. Add ECMatrix-511 to fresh media at a final concentration of 0.25 μg/cm2 (for example: for one well of a 6-well plate add 5 uL of the 0.5 mg/mL stock solution).
3. Add cells to the ECMatrix-511/Media and plate the cells at desired density.
Precoating Method
1. Dilute the 0.5 mg/mL stock solution with sterile PBS to achieve a 2.5 ug/mL working solution.
2. Coat dishes with ECMatrix-511 at 0.25 μg/cm2 (for example, for one well of a 6-well plate add 1 mL of the 2.5 μg/mL working solution).
3. Incubate for 1 hour at 37°C, 3 hours at room temperate or overnight at 4°C.
4. Before use, remove remaining fluid from the coated surface (do not rinse).
5. Detach cells into small clumps using Accutase.
6. Plate the cells at desired density.
Note: Do not allow the plates to dry, briefly spin down all liquids in the tube before use, avoid repeated freeze-thaw cycles.
儲存和穩定性
ECMatrix-511 E8 Laminin Substrates should be stored at 2-8°C. Avoid multiple freeze-thaw cycles and protect from light.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
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