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Key Documents

ABS204

Sigma-Aldrich

磷酸化-PDHE1-A I 型 (Ser293) 抗体

from rabbit, purified by affinity chromatography

别名:

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

物種活性(以同源性預測)

primate (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

phosphorylation (pSer293)

基因資訊

human ... PDHA1(5160)

一般說明

在许多生物体内,丙酮酸脱氢酶(PDH)复合物在有氧途径中催化丙酮酸整体不可逆地转化为乙酰辅酶A和CO2。这种复合物也是心脏底物选择的关键调节因子。它包含丙酮酸脱氢酶(E1或I型PDHE1-A)、二氢硫辛酰胺乙酰转移酶(E2)和硫辛酰胺脱氢酶(E3)三种酶组分的多个拷贝。PDH通过丙酮酸脱氢酶激酶(PDK)介导的磷酸化和反馈抑制调节。

特異性

该抗体可识别在Ser293处磷酸化的I型PDHE1-A。

免疫原

KLH偶联线性肽,对应于在Ser293处磷酸化的人I型PDHE1-A。
表位:磷酸化Ser293

應用

免疫细胞化学分析:代表性批次抗体的1:500稀释液在未经处理和经DCA处理的HEK293细胞中检测到磷酸化PDHE1-A I型(Ser293)。
可使用这种磷酸化I型PDHE1-A(Ser293)抗体检测磷酸化I型PDHE1-A(Ser293),该抗体经验证可用于WB、IC & IP。
研究子类别
葡萄糖/糖原代谢
研究类别
代谢

品質

通过蛋白质印迹法对未经处理和经DCA处理的HEK293细胞裂解液进行了评估。

蛋白质印迹分析:该抗体的1:10,000稀释液在未经处理和经DCA处理的HEK293细胞裂解液中检测到磷酸化I型PDHE1-A(Ser293)。

標靶描述

观测值〜43 kDa

外觀

亲和纯化
纯化的兔多克隆抗体,溶于含有0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。

儲存和穩定性

自收到之日起,在2-8°C条件下可稳定保存1年。

分析報告

对照
未经处理和经DCA处理的HEK293细胞裂解液

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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