推荐产品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human
物種活性(以同源性預測)
primate (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pSer293)
基因資訊
human ... PDHA1(5160)
一般說明
在许多生物体内,丙酮酸脱氢酶(PDH)复合物在有氧途径中催化丙酮酸整体不可逆地转化为乙酰辅酶A和CO2。这种复合物也是心脏底物选择的关键调节因子。它包含丙酮酸脱氢酶(E1或I型PDHE1-A)、二氢硫辛酰胺乙酰转移酶(E2)和硫辛酰胺脱氢酶(E3)三种酶组分的多个拷贝。PDH通过丙酮酸脱氢酶激酶(PDK)介导的磷酸化和反馈抑制调节。
特異性
该抗体可识别在Ser293处磷酸化的I型PDHE1-A。
免疫原
KLH偶联线性肽,对应于在Ser293处磷酸化的人I型PDHE1-A。
表位:磷酸化Ser293
應用
免疫细胞化学分析:代表性批次抗体的1:500稀释液在未经处理和经DCA处理的HEK293细胞中检测到磷酸化PDHE1-A I型(Ser293)。
可使用这种磷酸化I型PDHE1-A(Ser293)抗体检测磷酸化I型PDHE1-A(Ser293),该抗体经验证可用于WB、IC & IP。
研究子类别
葡萄糖/糖原代谢
葡萄糖/糖原代谢
研究类别
代谢
代谢
品質
通过蛋白质印迹法对未经处理和经DCA处理的HEK293细胞裂解液进行了评估。
蛋白质印迹分析:该抗体的1:10,000稀释液在未经处理和经DCA处理的HEK293细胞裂解液中检测到磷酸化I型PDHE1-A(Ser293)。
蛋白质印迹分析:该抗体的1:10,000稀释液在未经处理和经DCA处理的HEK293细胞裂解液中检测到磷酸化I型PDHE1-A(Ser293)。
標靶描述
观测值〜43 kDa
外觀
亲和纯化
纯化的兔多克隆抗体,溶于含有0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
儲存和穩定性
自收到之日起,在2-8°C条件下可稳定保存1年。
分析報告
对照
未经处理和经DCA处理的HEK293细胞裂解液
未经处理和经DCA处理的HEK293细胞裂解液
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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