ABS1515

Anti-phospho NFkB p52, (Ser222) Antibody

from rabbit, purified by affinity chromatography

• 别名: Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10, Nuclear factor NF-kappa-B p52 subunit
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• 技術: western blot: suitable
• NCBI登錄號: NP_002493
• UniProt登錄號: Q00653
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer222)
• 基因資訊: human ... NFKB2(4791)

说明

一般說明

The p52 NF-kB subunit is induced through activation of the alternative (noncanonical) NF-kB pathway in response to a range of stimuli such as lymphotoxin-b, BAFF and CD40, as well as DNA damage. The p52 formation results from proteolytic processing of its precursor protein p100 as a consequence of IKKalpha-mediated phosphorylation. The p52 is most commonly found as a heterodimer with RelB, although other dimer combinations have been identified by ChIP analysis. Ser222 of NF-kappa B p52 is phosphorylated by the kinase GSK3beta. Phosphorylation at S222 appears to regulate not only p52 partner dimerization but also the p52 dependent activation or repression of Skp2 gene expression.

免疫原

Epitope: RHD domain

KLH-conjugated linear peptide corresponding to the RHD domain of human phospho NFkB p52, (Ser222).

應用

Anti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected phospho NFkB p52, (Ser222) in CD40L and NTL cell lysate (Neil D. Perkins, Newcastle University).

品質

Evaluated by Western Blotting in cell lysate U2OS transfected with p52 WT.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho NFkB p52, (Ser222) in 10 µg of cell lysate U2OS transfected with p52 WT.

標靶描述

~52 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1