ABE262

Anti-HDAC4 Antibody

serum, from rabbit

• 别名: Histone deacetylase 4, HD4
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 技術: ChIP: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_006028
• UniProt登錄號: P56524
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HDAC4(9759)

说明

一般說明

Histone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Transcription is controlled in part by the dynamic acetylation and deacetylation of histone proteins. Histone deacetylases (HDACs) are recruited to specific promoters or chromosomal domains by association with DNA-binding proteins. HDAC4 is a histone deacetylase which deacetylates lysines on the core histones. HDAC4 accumulates in the nuclei of myotubes, suggesting involvement in muscle differentiation. HDAC4 does not bind DNA directly but interacts with the myocyte enhancer factors MEF2A, MEF2C, and MEF2D. HDAC4 also interacts with the breast cancer associated transcriptional repressor PLU-1/JARID1B, the Nr2c1/TR2 nuclear receptor, as well as the 14-3-3 chaperone protein, in a phosphorylation dependent manner.

特異性

This antibody recognizes HDAC4 at the C-terminus.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human HDAC4.

應用

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HDAC4 in HeLa and A431 cells.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

Use Anti-HDAC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP, ChIP to detect HDAC4 also known as Histone deacetylase 4, HD4.

品質

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected HDAC4 on 10 µg of HepG2 cell lysate.

標靶描述

~125 kDa observed

聯結

Replaces: 04-232

外觀

Unpurified

Rabbit polyclonal serum containing 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HepG2 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1