ABE1462

Anti-phospho LSD1 (Ser 112) Antibody

from rabbit, purified by affinity chromatography

• 别名: Lysine-specific histone demethylase 1A, Ser 112 phosphorylated, BRAF35-HDAC complex protein BHC110, Ser 112 phosphorylated, Flavin-containing amine oxidase domain-containing protein 2, Ser 112 phosphorylated
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), porcine (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• 技術: dot blot: suitable; western blot: suitable
• NCBI登錄號: NP_598633
• UniProt登錄號: Q6ZQ88
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer112 )
• 基因資訊: human ... KDM1A(23028)

说明

一般說明

Lysine-specific histone demethylase 1A (UniProt Q6ZQ88; also known as BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) is encoded by the Kdm1a (also known as Aof2, Kiaa0601, Lsd1) gene (Gene ID 99982) in murine species. The FAD+-dependent, lysine-specific demethylase (LSD) LSD1 is a component of the REST corepressor (CoREST) complexes that mediate gene repression by catalyzing the demethylation of mono- and di-methylated histone H3 Lys4 (H3K4me1/2). LSD1 is also involved in gene activation process associated with nuclear receptors through demethylation of H3K9me1/2. In addition, LSD1 is known to demethylate non-histone substrates, such as p53 and Dnmt1. LSD1 is reported to be phosphorylated on Ser112 by PKCalpha and thereby mediate PKCalpha signaling-dependent circadian clock regulation. Ser112 phosphorylation induces LSD1 interaction with CLOCK:BMAL1 to facilitate E-box-dependent transcriptional activation. Knockin mice bearing Lsd1(SA/SA) alleles coding for phosphorylation-defective S112A LSD1 mutant are reported to exhibit altered circadian rhythms in locomotor behavior with attenuated rhythmic expression of core clock genes and impaired circadian clock phase resetting.

特異性

The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by dot blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).

免疫原

Epitope: pSer112.

Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112.

應用

Anti-phospho LSD1 (Ser 112) Antibody is an antibody against phospho LSD1 (Ser 112) for use in Western Blotting, Dot Blot.

Dot Blot Analysis: A representative lot detected the immunogen peptide with phosphorylated Ser112 (equivalent to human pSer111), but not the corresponding peptide with non-phosphorylated Ser112 (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
Western Blotting Analysis: A representative lot detected phosphorylation induction of exogenously expressed human LSD1 upon PMA treatment of 24-hour starved HEK293T transfectants. Phosphorylated LSD1 was detected in the nuclear, but not cytosolic fraction and PKC inhibitor Go 6976 (Cat. No. 365250) co-treatment prevented PMA-induced LSD1 phosphorylation (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
Western Blotting Analysis: A representative lot detected a time-dependent LSD1 phosphorylation level in liver extracts from mice kept in a constantly dark (DD) cycle. Nuclear extracts from MEFs at different time points following circadian gene transcription induction by dexamethasone treatment likewise showed circadian-like oscillation of LSD1 phosphorylation level (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).

品質

Evaluated by Western Blotting in human LSD1-overexpressing HEK293T cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PMA-induced phosphorylation of exogenously expressed human LSD1 in 24-hour starved HEK293T transfectants. Lambda phosphatase treatment of the membrane prior to antibody probing completely abolished the target band detection.

標靶描述

~115 kDa observed. 92.90/95.16 kDa (human isoform 1/2), 92.85 (mouse), and 94.60 kDa (rat) calculated. Uncharacterized band(s) may appear in some lysates.

其他說明

Concentration: Please refer to lot specific datasheet.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable