推荐产品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat, rabbit, mouse, human
製造商／商標名
Chemicon®
技術
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... CACNA1C(775)
一般說明
Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to dhp compounds (Swiss Prot).
特異性
Recognizes alpha 1C subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Guinea pig (17/18 residues identical); human and rabbit (16/18 residues identical).
免疫原
Purified peptide (C)TTKI NMDDL QPSEN EDKS (CNC1) from 848-865 of alpha 1C subunit of rat brain voltage-gated calcium channel (Accession number P22002). Epitope location is in the intracellular loop between domains II and III.
應用
Alpha 1 subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunohistochemistry: 1:200 on rat brain sections.
Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions and protocols must be determined by the end user.
Immunohistochemistry: 1:200 on rat brain sections.
Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions and protocols must be determined by the end user.
Detect Calcium Channel using this Anti-Calcium Channel Antibody, Voltage Gated α 1C, validated for use in IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Signaling Neuroscience
Ion Channels & Transporters
Signaling Neuroscience
標靶描述
190 & 210 kDa
外觀
ImmunoAffinity Purified
Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
儲存和穩定性
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution add glycerol (ASC grade or better) at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
分析報告
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Timothy syndrome is associated with activity-dependent dendritic retraction in rodent and human neurons.
Krey, JF; Pasca, SP; Shcheglovitov, A; Yazawa, M; Schwemberger, R; Rasmusson, R; Dolmetsch, RE
Nature Neuroscience null
State-dependent signaling by Cav1.2 regulates hair follicle stem cell function.
Yucel, G; Altindag, B; Gomez-Ospina, N; Rana, A; Panagiotakos, G; Lara, MF; Dolmetsch, R; Oro, AE
Genes & Development null
Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway.
Dolmetsch, R E, et al.
Science (New York, N.Y.), 294, 333-339 (2001)
Wei Wang et al.
Molecular medicine reports, 14(4), 3848-3854 (2016-09-08)
Remodeling of atrial electrophysiology and structure is the primary feature of atrial fibrillation (AF). Evidence suggests that abnormalities in the expression levels of embryological cardiovascular development‑associated transcription factors, including Nkx2.5, are crucial for the development of AF. Rat atrial myocardial
Georgia Panagiotakos et al.
eLife, 8 (2019-12-24)
The syndromic autism spectrum disorder (ASD) Timothy syndrome (TS) is caused by a point mutation in the alternatively spliced exon 8A of the calcium channel Cav1.2. Using mouse brain and human induced pluripotent stem cells (iPSCs), we provide evidence that
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