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Merck
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AB3203

Sigma-Aldrich

Anti-Tubulin Antibody, δ 2

Chemicon®, from rabbit

别名:

Anti-α-tubulin, Tubulin antibody

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

sea urchin, echinoderm, plant

應無反應活性

Saccharomyces cerevisiae

物種活性(以同源性預測)

mammals

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... TUBD1(51174)

一般說明

Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form.

特異性

The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991).

SPECIES REACTIVITY:

Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).

免疫原

Peptide corresponding to the C-terminal of delta 2 tubulin.

應用

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin.
Western blot: 1:1,000

Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500

Optimal working dilutions must be determined by the end user.

外觀

Affinity purified immunoglobulin. Liquid in PBS.

儲存和穩定性

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Robert O'Hagan et al.
Current biology : CB, 21(20), 1685-1694 (2011-10-11)
Posttranslational modifications (PTMs) such as acetylation, detyrosination, and polyglutamylation have long been considered markers of stable microtubules and have recently been proposed to guide molecular motors to specific subcellular destinations. Microtubules can be deglutamylated by the cytosolic carboxypeptidase CCP1. Loss
Tracy Chong et al.
BMC developmental biology, 11, 69-69 (2011-11-15)
The freshwater planarian Schmidtea mediterranea exhibits two distinct reproductive modes. Individuals of the sexual strain are cross-fertilizing hermaphrodites with reproductive organs that develop post-embryonically. By contrast, individuals of the asexual strain reproduce exclusively by transverse fission and fail to develop
Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration.
Ghosh-Roy, A; Goncharov, A; Jin, Y; Chisholm, AD
Developmental Cell null
Epigenetic characterization of the vegetative and floral stages of azalea buds: dynamics of DNA methylation and histone H4 acetylation.
Monica Meijon,Luis Valledor,Estrella Santamaria,Pilar S Testillano,Ma Carmen Risue?o et al.
Journal of Plant Physiology null
Isolation rearing induces recognition memory deficits accompanied by cytoskeletal alterations in rat hippocampus.
Bianchi, M, et al.
The European Journal of Neuroscience, 24, 2894-2902 (2006)

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