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Key Documents

AB16301

Sigma-Aldrich

Anti-Nitric Oxide Synthase III Antibody

Chemicon®, from rabbit

别名:

NOS III, eNOS

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... NOS3(4846)

特異性

Reactive with human NOS-III (eNOS). This immunogen sequence is 100% homologous in human, bovine, rat, and mouse, but reactivity has not been tested.

免疫原

Synthetic peptide corresponding to amino acids 596-610 of human endothelial NOS, coupled to KLH.

應用

Anti-Nitric Oxide Synthase III Antibody detects level of Nitric Oxide Synthase III & has been published & validated for use in WB & IC.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Western Blot: 5 μg/mL using chemiluminescent detection. Detects ~135-140kDa band in endothelial cell preparations; Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 5-10 μg/mL

Optimal working dilutions must be determined by end user.

外觀

Affinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.

儲存和穩定性

Store at 2-8°C in undiluted aliquots for up to 6 months.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Fahima Syeda et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 166(1), 207-214 (2013-06-04)
The ability to regulate vascular tone is an essential cardiovascular control mechanism, with nitric oxide (NO) assumed to be a ubiquitous smooth muscle relaxant. However, the literature contains reports of vasoconstrictor, vasodilator and no response to nitroergic stimulation in non-mammalian
The interactive effects of hypoxia and nitric oxide on catecholamine secretion in rainbow trout (Oncorhynchus mykiss).
McNeill, B; Perry, SF
The Journal of Experimental Biology null
Morphological support for paracrine inhibition of gastric acid secretion by nitric oxide in humans
Berg, A, et al
Scandinavian Journal of Gastroenterology, 36, 1016-1021 (2001)
Angiotensin II-induced contraction is attenuated by nitric oxide in afferent arterioles from the nonclipped kidney in 2K1C.
Helle, F; Hultstrom, M; Skogstrand, T; Palm, F; Iversen, BM
American Journal of Physiology: Renal Physiology null

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