AB10525

Anti-EBF-3 Antibody

from rabbit, purified by affinity chromatography

• 别名: Transcription factor COE3, Early B-cell factor 3, EBF-3, Olf-1/EBF-like 2, O/E-2, OE-2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), human (based on 100% sequence homology)
• 技術: dot blot: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NP_001106886
• UniProt登錄號: O08791
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EBF3(253738) ; mouse ... Ebf3(13593)

说明

一般說明

The family of Early B-cell Factor (EBF) or Collier/Olf-1/EBF (COE) proteins, EBF1, EBF2, and EBF3, are ubiquitous zinc-binding transcription factors found in many species, including human, Drosophila melanogaster, and Caenorhabditis elegans. EBF proteins are capable of forming homo- and heterodimers that bind to DNA at specific sites that include the sequence 5′-ATTCCCNNGGGAATT-3′. EBF1, the prototypical member of this family, may employ epigenetic mechanisms, such as DNA methylation and chromatin remodeling, to regulate genes involved in B-cell development such as Pax5. The second member of this family EBF2 is involved in bone development, adipogenesis, and CNS development, processes which may also hinge on EBF1. EBF3 has been characterized as a tumor suppressor protein, due to its role in regulating multiple genes such as those for cyclins and CDKs which control cell growth, differentiation, and apoptosis. However, emerging evidence indicates that all EBF proteins may play a role in the development of various cancers.

特異性

This antibody recognizes EB-3 but not EB-1 or EB-2.

免疫原

KLH-conjugated linear peptide corresponding to mouse EBF-3.

應用

Anti-EBF-3 Antibody is an antibody against EBF-3 for use in Western Blotting, IHC(P), Immunofluorescence, Dot Blot.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blot Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF-3. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-3 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)

Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-3 (1:100 dilution). No cross reactivity to peptides for EBF-1 & EBF-2 were observed.

品質

Evaluated by Western Blot in mouse E16 brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected EBF-3 in 10 µg of mouse E16 brain tissue lysate.

標靶描述

~72 kDa observed. An uncharacterized band may appear at ~42, ~55, and ~108 kDa in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Mouse E16 brain tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable