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形狀
suspension (Homogeneous)
品質等級
製造商／商標名
Calbiochem®
儲存條件
do not freeze
雜質
≤2500 cells/mL Viable cells
顏色
milky
容量
≥2.0 mg(human IgG/100 mg cells)
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
PANSORBIN细胞是加热灭活的，福尔马林固定的金黄色葡萄球菌细胞，其具有蛋白A的外壳，并已通过Kessler的方法进行了腌制。可用于B淋巴细胞的促有丝分裂刺激和免疫沉淀。
加热灭活的，福尔马林固定的金黄色葡萄球菌细胞（Cowan I菌株），含有高细胞表面密度的蛋白A，并已通过Kessler方法进行了腌制。由于蛋白A与IgG Fc结构域的高亲和相互作用，可用作固相IgG结合试剂。当抗体为人（IgG1，IgG2，IgG4），兔IgG（全部同种型）或小鼠（IgG2a，IgG2b，IgG3）时，PANSORBIN细胞的工作效果最佳。最常见的应用包括免疫沉淀和B淋巴细胞的促有丝分裂刺激。
警告
毒性：标准处理（A）
外觀
以含金黄色葡萄球菌细胞的PBS、0.1%NaN₃、pH值7.2的10%混悬剂形式提供。
其他說明
Kierszenbaum, F., et al. 1991.Immunology74, 317.
Meikle, P.J., et al. 1991.J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989.Biochem.Biophys.Res. Commun.159, 1368.
Murakami, H., et al. 1988.Biochem.J.256, 917.
Kessler, S.W.1975.J. Immunol. 115, 1617.
Meikle, P.J., et al. 1991.J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989.Biochem.Biophys.Res. Commun.159, 1368.
Murakami, H., et al. 1988.Biochem.J.256, 917.
Kessler, S.W.1975.J. Immunol. 115, 1617.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Acute Tox. 4 Oral
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Leah S Bernstein et al.
Methods in molecular biology (Clifton, N.J.), 237, 195-204 (2003-09-23)
Palmitoylation refers to the covalent attachment of a 16-carbon fatty acid to cysteine residues of proteins. This modification occurs on many intracellular signaling proteins including regulators of G protein signaling proteins (RGS). Palmitoylation mediates the interaction of proteins with membranes
T A Bunch et al.
Nucleic acids research, 17(23), 9761-9782 (1989-12-11)
Genes producing antisense RNA are becoming important tools for the selective inhibition of gene expression. Experiments in different biological systems, targeting different mRNAs have yielded diverse results with respect to the success of the technique and its mechanism of action.
J R Faust et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(17), 5205-5209 (1982-09-01)
UT-1 cells are a clone of Chinese hamster ovary cells that were selected to grow in the presence of compactin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase [mevalonate: NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34]. These cells have 100- to 1,000-fold more
Derek Walsh et al.
Genes & development, 20(4), 461-472 (2006-02-17)
Recruitment of the 40S ribosome to the 5' end of a eukaryotic mRNA requires assembly of translation initiation factors eIF4E, the cap-binding protein, together with eIF4A and eIF4G into a complex termed eIF4F. While the translational repressor 4E-BP1 regulates binding
M Achison et al.
The Journal of biological chemistry, 276(5), 3167-3174 (2000-12-09)
Collagen fibers or a glycoprotein VI-specific collagen-related peptide (CRP-XL) stimulated tyrosine phosphorylation of the focal adhesion kinase, p125(fak) (FAK), in human platelets. An integrin alpha(2)beta(1)-specific triple-helical peptide ligand, containing the sequence GFOGER (single-letter nomenclature, O = Hyp) was without effect.
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