405210
ID-8
A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF
别名:
ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
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About This Item
推荐产品
品質等級
化驗
≥95% (HPLC)
形狀
powder
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
protect from light
顏色
yellow
溶解度
DMSO: 50 mg/mL
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.
包裝
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
其他說明
Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
Tomoyuki Miyabayashi et al.
Bioscience, biotechnology, and biochemistry, 72(5), 1242-1248 (2008-05-08)
Embryonic stem cells (ESCs), which have characteristics such as self-renewal, indefinite proliferation, and pluripotency, are thought to hold great promise for regenerative medicine. ESCs are generally cultured on mouse embryonic fibroblast (MEF) or MEF conditioned medium (MEF-CM). However, for therapeutic
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