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Merck
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主要文件

33-125

Sigma-Aldrich

PIP5-Kinase HTRF Assay

The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.54

品質等級

100

物種活性

mouse, human

製造商／商標名

HTRF®
Upstate®

技術

activity assay: suitable (kinase)

NCBI登錄號

NM_001135636.1
NM_001195733.1

檢測方法

fluorometric (HTRF)

運輸包裝

dry ice

應用

Research Category
Apoptosis & Cancer
Research Sub Category
Oncology
The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.
Used to detect/quantify: PIP5 Kinase

生化／生理作用

Target Sub-Family: PI3K/PI4K

包裝

Kit capacity: 384 wells

成分

Reaction Buffer (4X)

DM A Detection Reagent

DM C Detection Reagent

DM E Detection Reagent

Phosphatidylinositol 4-phosphate (1mM)

Phosphatidylserine (1mM)

Stop Reagent D

儲存和穩定性

12 months at -70°C

其他說明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法律資訊

HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10-13 - German Storage Class 10 to 13

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Yuxun Zhang et al.
The Journal of biological chemistry, 292(24), 10239-10249 (2017-05-02)
SIRT5 is a lysine desuccinylase known to regulate mitochondrial fatty acid oxidation and the urea cycle. Here, SIRT5 was observed to bind to cardiolipin via an amphipathic helix on its N terminus. In vitro, succinyl-CoA was used to succinylate liver
Marco Spinazzi et al.
Nature protocols, 7(6), 1235-1246 (2012-06-02)
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investigating mitochondrial function in several situations, including mitochondrial disorders, diabetes, cancer, aging and neurodegeneration, as well as for many toxicological assays. Muscle is the most commonly analyzed tissue

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