2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• 别名: Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• CAS号: 9001-15-4
• EC 号: 2.7.3.2 (BRENDA, IUBMB)
• MDL號碼: MFCD00130876
• 分類程式碼代碼: 12352202

属性

• 生物源: rabbit skeletal muscle
• 品質等級: 200
• 形狀: lyophilized
• 比活性: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic)
• 溶解度: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• 異物活動: ATPase, hexokinase, myokinase <0.001%
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

Native creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

重構

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

其他說明

Bessman, S.P. 1987. Anal. Biochem.161, 519.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable