218800

Caspase-7, Human, Recombinant, E. coli

Caspase-7, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• 别名: Mch3, ICE-LAP3
• 分類程式碼代碼: 12352202

属性

• 重組細胞: expressed in E. coli
• 品質等級: 100
• 形狀: lyophilized
• 比活性: ≥15,000 units/mg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −70°C

说明

一般說明

M.W. 12,000 and 20,000 (heterodimer).

Recombinant, human caspase-7 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA (Cat. No. 235400) per hour at 37°C, pH 7.2.

重構

Following reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.

Reconstitute to 1 unit/µl in PBS, 15% glycerol.

其他說明

Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable