207005

Calcineurin Assay Kit, Colorimetric

• 别名: Colorimetric, PP2B Assay Kit
• 分類程式碼代碼: 12161503
• NACRES: NA.84

属性

• 用途: sufficient for 96 tests
• 品質等級: 100
• 製造商／商標名: Calbiochem^®
• 包裝: pkg of 96-well plate(s)
• 儲存條件: OK to freeze
• 檢測方法: colorimetric
• 運輸包裝: dry ice
• 儲存溫度: −70°C

说明

一般說明

A colorimetric assay kit for measuring calcineurin (PP2B) activity and for inhibitor screening. The assay is performed using RII Phosphopeptide Substrate (Cat. No. 207008).

包裝

1 kit in Glass bottle

成分

Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS

規格

Assay time: 3.5 h

儲存和穩定性

Store all components except the 96-Well Plate (room temperature) at -70°C for the highest stability. In particular, calcineurin must be handled with utmost care in order to retain its maximal enzymatic activity. Thaw it quickly in a water bath, maintained at room temperature, or by rubbing between fingers, then immediately store on an ice bath. Any remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, we recommend to aliquot the calcineurin into separate tubes and then store at -70°C.

其他說明

Mondragon, A. et al. 1997. Biochemistry 36, 4934.
Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS05
• 訊號詞: Warning
• 危險聲明: H290
• 防範說明: P234 - P390
• 危險分類: Met. Corr. 1
• 儲存類別代碼: 8A - Combustible corrosive hazardous materials