196810

Bax-Inhibiting Peptide, V5

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection.

• 别名: Bax-Inhibiting Peptide, V5, BIP-V5, H-VPMLK-OH
• 经验公式（希尔记法）: C₂₇H₅₀N₆O₆S
• 分子量: 586.79
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: lyophilized solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 顏色: white
• 溶解度: water: 1 mg/mL
• 運輸包裝: wet ice
• 儲存溫度: −20°C

说明

一般說明

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).

A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).

生化／生理作用

Cell permeable: yes

Primary Target
Bax mediated apoptosis

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50-200 µM against Bax mediated apoptosis

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

序列

H-Val-Pro-Met-Leu-Lys-OH

重構

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.

其他說明

Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable