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Merck
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Key Documents

1.46689

Millipore

ReadyPlate Chromocult® Coliform Agar

To detect Escherichia coli and coliform bacteria, plate diam. 90 mm, box of 20 plates

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About This Item

分類程式碼代碼:
41106217
NACRES:
NA.74

agency

ISO 9308

品質等級

無菌

sterile

形狀

plate (ready-to-use)

特點

ready-to-use

包裝

box of 20 plates

製造商/商標名

ReadyPlate

板直徑

90 mm

應用

food and beverages
microbiology
water monitoring

儲存溫度

15-25°C

適合性

Escherichia coli
coliforms

一般說明

ReadyPlate agar plates are stackable specially designed plates that helps increase the safety during handling, transport and incubation. ReadyPlate agar plates are of standard 90mm diameter. Chromocult® Coliform Agar (CCA) is a selective and differential chromogenic culture medium. It is a standard medium in water testing for simultaneous detection and enumeration of coliform and E.coli. It is also the base medium recommended for testing drinking water as per ISO 9309-1:2014

應用

Chromocult® Coliform Agar is used for the enumeration of Escherichia coli and coliform bacteria in food and drinking water, disinfected pool water and finished water from treatment plants

分析報告

The interaction of selected peptones, pyruvate, sorbitol and phosphate buffer promotes rapid colony growth, even for sub-lethally injured coliform bacteria. The growth of Gram-positive bacteria as well as certain Gram-negative bacteria is inhibited by the presence of Tergitol 7 which has no negative effect on the growth of coliform bacteria. Further, the combination of two chromogenic substrates permits the simultaneous detection of coliform bacteria and E. coli.
Coliform bacteria detection: The substrate Salmon-GAL is used for the detection of β-D-galactosidase activity, which is characteristic for coliform bacteria. This interaction results in a pink to red color of the coliform colonies.
E. coli detection: The substrate X-glucuronide is used for the detection of β-D-glucuronidase activity. E. coli cleaves both Salmon-GAL and X-glucuronide, so that positive colonies take on a dark-blue to violet color. These are easily distinguished from other coliform colonies, which have a pink to red color. Some E. coli (3-4%) are ß-D-glucuronidase-negative and appear as pink to red colonies.
Accompanying flora appears as colorless colonies, except for some organisms, which possess β-D glucuronidase activity. These colonies appear light blue to turquoise in color.

法律資訊

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
READYPLATE is a trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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