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化驗
≥95% (cellulose acetate electrophoresis)
形狀
powder
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
雜質
≤0.02% fatty acids
≤3% ash
≤5% moisture
顏色
white to light yellow
light yellow to light brown
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
牛血清白蛋白(BSA、组分V、不含脂肪酸)适用于免疫诊断分析。它设计用于血清学检测、放射免疫测定和激素反应研究。它也适用于结合和运输研究。BSA采用冷乙醇提取法(Cohn法)制备。pH值:6.8-7.4。
牛血清白蛋白(BSA)是一种非糖基化球状α-螺旋蛋白,属于血清白蛋白家族。它由三个结构域组成,每个结构域有两个子结构域,并且具有17个二硫键。
應用
牛血清白蛋白(组分V、不含脂肪酸)已用作:
- 一种组分以用于各种细胞免疫电子显微镜(IEM)的阻断缓冲液
- 线粒体隔离缓冲液(MIB)和线粒体测定液(MAS),以捕获脂肪组织均质化过程中释放的游离脂肪酸
- 一种阻断缓冲液,以对食管上皮(EE)进行免疫荧光全旦染色
生化/生理作用
牛血清白蛋白(BSA)是细胞培养基中最重要的成分,有助于胚胎干细胞(hESC)的分化。它有助于药物、激素和脂肪酸的运输。BSA也被用作酶联免疫吸附试验(ELISA)的阻断剂。
警告
毒性:标准处理(A)
準備報告
可能需要长达24小时才能完全溶解。
重構
在复溶后存放于冰箱(4°C)。可能添加0.1% NaN₃作为防腐剂。储备溶液在4°C下可稳定保存3个月。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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