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Merck
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主要文件

1.10338

Millipore

Fractogel EMD螯合物(M)

purified by immobilized metal affinity chromatography (IMAC)

别名:

Fractogel® EMD螯合物(M)

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About This Item

分類程式碼代碼:
41115711

ligand

iminodiacetic acid

品質等級

描述

for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

無菌

sterile (Caustic Stable)

形狀

resin

純化經由

immobilized metal affinity chromatography (IMAC)

製造商/商標名

Fractogel®

參數

8 bar max. pressure
~240 cm/hr flow rate

基質活性組

methacrylate

40-90 μm

容量

~60 mg binding capacity( lysozyme/ml of resin)

轉變溫度

flash point 35 °C (calculated)

應用

recombinant protein

分離技術

affinity

儲存溫度

2-30°C

一般說明

Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand

特點和優勢

Fractogel® EMD Chelate (M) enables users to:

  • Efficiently purify histidine-tagged recombinant proteins
  • Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
  • Ensure timely and successful startup and operation
  • Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
  • Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime

包裝

  • 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
  • 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
  • 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
  • 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L

Plastic bottle

分析報告

Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

95.0 °F

閃點(°C)

35 °C


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