推荐产品
ligand
(Trimethylammoniumethyl)
品質等級
描述
strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
無菌
sterile (Caustic Stable)
形狀
resin
特定分析物
MAbs
參數
180 cm/hr flow rate
8 bar max. pressure
基質活性組
methacrylate
平均粒徑
40-90 μm
容量
180 mg binding capacity (BSA/mg of resin)
轉變溫度
flash point 35 °C (calculated)
密度
1.430 g/cm3 at 20 °C
體積密度
1000 kg/m3
應用
recombinant protein
分離技術
anion exchange
strong anion exchange
儲存溫度
2-30°C
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一般說明
Strong anion exchange Fractogel® resin featuring the tentacle technology with a trimethylammoniumethyl functional group for purification of acidic and neutral proteins and peptides from multiple sources, DNA and virus removal, plasmid purification, vaccine purification, and more
特點和優勢
Fractogel® EMD TMAE Hicap (M) enables users to:
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
- Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
包裝
- 1.10316.0100: Fractogel® EMD TMAE Hicap (M) Resin 100ml
- 1.10316.0010: Fractogel® EMD TMAE Hicap (M) Resin 10ml
- 1.10316.0500: Fractogel® EMD TMAE Hicap (M) Resin 500ml
- 1.10316.5000: Fractogel® EMD TMAE Hicap (M) Resin 5L
Plastic bottle
分析報告
Appearance: Milky turbid suspension, free from impurities (foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 160 - 200 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 160 - 200 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin
法律資訊
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
95.0 °F
閃點（°C）
35 °C
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