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Merck
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Key Documents

1.09270

Sigma-Aldrich

Papanicolaou′s solution 3c Polychromatic solution EA 65

for cytology

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About This Item

分類程式碼代碼:
41116113

品質等級

形狀

liquid

IVD

for in vitro diagnostic use

pH值

5.8 (20 °C in H2O)

轉變溫度

flash point 15 °C

密度

0.82 g/cm3 at 20 °C

應用

clinical testing
diagnostic assay manufacturing
hematology
histology

儲存溫度

15-25°C

一般說明

Papanicolaou′s solution 3c polychromatic solution EA 65 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou′s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Papanicolaou′s solution 1a Harris hematoxylin solution, (Product number 1.09253) and Papanicolaou′s solution 1b Hematoxylin solution S, (Product number 1.09254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou′s solution 2b Orange II solution, Product number 1.106887 or Papanicolaou′s solution 2a Orange G solution (OG6), Product number 1.06888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou′s solution 3a polychromatic solution EA 31, (Product number 1.09271), Papanicolaou′s solution 3b polychromatic solution EA 50, (Product number 1.09272), Papanicolaou′s solution 3c polychromatic solution EA 65 (Product number 1.09270), o Papanicolaou′s solution 3d polychromatic solution EA 65, (Product number 1.09269). The polychrome solution is used for demonstration of differentiation of squamous cells. The Papanicolaou′s solution 3c polychromatic solution EA 65 will result in a staining with a light-brown to red staining result.
The 100 ml bottle provides 300 - 400 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

應用

Cytoplasm staining esp. for non-gynecological specimen, color effect light red

分析報告

Suitability for microscopy (Vaginal smear)passes testNucleiblue to dark violetCytoplasmred

象形圖

FlameExclamation mark

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

59.0 °F

閃點(°C)

15 °C


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