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生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat, human
物種活性(以同源性預測)
mouse
技術
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... CARM1(10498)
mouse ... Carm1(59035)
一般說明
CARM1(与共激活因子相关的精氨酸甲基转移酶1)是一种精氨酸N-甲基转移酶,可催化甲基从S-腺苷-L-蛋氨酸转移至蛋白质中的精氨酸残基。 CARM1目标包括Arg17处的组蛋白H3和Arg2142处的p300甲基化。基因激活后,CARM1与来自p300和p160家族的组蛋白乙酰转移酶一起被募集到启动子。在单核细胞炎症刺激过程中,CARM1与p300一起充当NF-kB的共激活因子。它负责在发育中的胚泡中将细胞移向内部细胞团。CARM1在雄激素受体中也起着重要作用,并可能在前列腺癌进展中起作用。
特異性
可识别CARM1,Mr ~60 kDa。
免疫原
含有序列(CSPMSIPTNTMHYGS)的KLH偶联的合成肽,对应于人CARM1的氨基酸595-608。
表位:氨基酸595-608
應用
抗CARM1抗体是一种兔多克隆抗体,用于检测CARM1(也称为蛋白质精氨酸N-甲基转移酶4,共激活因子相关的精氨酸甲基转移酶1),&已在WB中得到验证。
蛋白质印迹分析:
该批次的1 μg/mL稀释液在HeLa核提取物中检测到CARM1。(图A)。
该批次的1 μg/mL稀释液在HeLa核提取物中检测到CARM1。(图A)。
標靶描述
~60 kDa
外觀
形式:纯化
通过肽亲和层析纯化。在0.1M Tris-甘氨酸(pH 7.4),15 mM NaCl和0.05% 叠氮化钠中以1 mg/ml提供。
分析報告
对照
包括阳性抗原对照:目录号12-309,HeLa核提取物。将等体积的Laemmli样品还原性缓冲液加至10 μL裂解液中,煮沸5分钟以对制剂进行还原。 在微凝胶每个泳道上样20 μg经还原的裂解液。
包括阳性抗原对照:目录号12-309,HeLa核提取物。将等体积的Laemmli样品还原性缓冲液加至10 μL裂解液中,煮沸5分钟以对制剂进行还原。 在微凝胶每个泳道上样20 μg经还原的裂解液。
其他說明
浓度:请参考批次特异性浓缩物的分析证书。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Nature, 445(7124), 214-218 (2007-01-12)
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The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors
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