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生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
mouse, human
製造商／商標名
Upstate®
技術
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
phosphorylation (pThr246)
基因資訊
human ... AKT1S1(84335)
mouse ... Akt1S1(67605)
一般說明
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
特異性
Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.
免疫原
Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).
應用
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
品質
Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
標靶描述
Approx. 40 kDa
外觀
Format: Purified
Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.
分析報告
Control
PDGF stimulated NIH3T3 cells.
PDGF stimulated NIH3T3 cells.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
Inositol polyphosphate multikinase is a physiologic PI3-kinase that activates Akt/PKB.
Maag, D; Maxwell, MJ; Hardesty, DA; Boucher, KL; Choudhari, N; Hanno, AG; Ma, JF; Snowman et al.
Proceedings of the National Academy of Sciences of the USA null
Regulation of Akt during torpor in the hibernating ground squirrel, Ictidomys tridecemlineatus.
McMullen DC, Hallenbeck JM
Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology null
Paul M Titchenell et al.
Cell metabolism, 23(6), 1154-1166 (2016-05-31)
During insulin-resistant states such as type II diabetes mellitus (T2DM), insulin fails to suppress hepatic glucose production (HGP) yet promotes lipid synthesis. This metabolic state has been termed "selective insulin resistance" to indicate a defect in one arm of the
Noriko Oshiro et al.
The Journal of biological chemistry, 289(5), 2658-2674 (2013-12-18)
Activation of mammalian target of rapamycin complex 1 (mTORC1) by amino acids is mediated in part by the Rag GTPases, which bind the raptor subunit of mTORC1 in an amino acid-stimulated manner and promote mTORC1 interaction with Rheb-GTP, the immediate
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