07-2184

Anti-GAPDH Antibody

rabbit polyclonal

• 别名: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• 無性繁殖: polyclonal
• application: IP; WB; inhibition assay
• 物種活性: human
• 技術: immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable

属性

• 产品名称: Anti-acetyl-GAPDH (Lys160) Antibody, 0.5 mg/mL, from rabbit
• 生物源: rabbit
• 品質等級: 100
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: canine (based on 100% sequence homology), horse (based on 100% sequence homology), bovine (based on 100% sequence homology), sheep (based on 100% sequence homology), feline (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), mouse (based on 100% sequence homology)
• 濃度: 0.5 mg/mL
• 技術: immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_002037
• UniProt登錄號: P04406
• 運輸包裝: wet ice
• 目標翻譯後修改: acetylation (Lys160)
• 基因資訊: human ... GAPDH(2597)

说明

一般說明

Acetyl-GAPDH (Lys160), also known as Glyceraldehyde-3-phosphate dehydrogenase (GAPDH(lys160), or Peptidyl-cysteine S-nitrosylase GAPDH, and encoded by the gene GAPDH/GAPD, is an interesting multifunctional metabolic enzyme that plays a critical role in glycolysis but also many other important nuclear and cell functions such as transcription, RNA transport, DNA replication and even apoptosis and cell death. In glycolysis, GAPDH is a key enzyme that catalyzes the first step in the pathway by converting G3P into 3-phospho-D glyceroyl phosphate. An example of GAPDH’s regulation of gene transcription as a component of a GAIT complex, which selectively inhibits the activation of various gamma interferon induced inflammation responses. The protein-protein interactions of GAPDH are at least partially controlled by acetylation. For instance, transport of GAPDH into the nucleus is dependent upon acetylation at lysines 117, 227, and 251, and acetylation at lysine 183 governs co-factor interactions. Acetylation of GAPDH at lysine 160 ultimately is involved in p53 mediated cell death.

免疫原

Epitope: 0

KLH-conjugated linear peptide corresponding to the human GAPDH acetylated at Lys160.

應用

Peptide Inhibition Analysis: 1 µg/mL from a representative lot detected acetyl GAPDH (Lys60) in LPS/IFNgamma in RAW264.7 nuclear extract.

Immunoprecipitation Analyis: A representative lot immunoprecipitated acetyl GAPDH (Lys60) from LPS/IFNgamma treated RAW264.7 cell lysate (Prof. Akira Sawa, Johns Hopkins University, Baltimore.).

Research Category
Metabolism

Research Sub Category
Insulin/Energy Signaling

This Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).

品質

Evaluated by Western Blotting in untreated and LPS/IFNgamma treated RAW264.7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl GAPDH (Lys60) in LPS/IFNgamma treated RAW264.7 cell lysate and not in untreated RAW264.7 cell lysate.

標靶描述

~37 kDa observed

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable