07-169-I

Anti-iPLA2 Antibody

from rabbit, purified by affinity chromatography

• 别名: iPLA2, 85/88 kDa calcium-independent phospholipase A2 (EC:3.1.1.4), CaI-PLA2, Group VI phospholipase A2, GVI PLA2, Intracellular membrane-associated calcium-independent phospholipase A2 beta, iPLA2-beta, Patatin-like phospholipase domain-containing prote
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: hamster (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• 技術: immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001186491
• UniProt登錄號: O60733
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PLA2G6(8398)

说明

一般說明

Phospholipase A2 (PLA2) enzymes constitute the largest class of phospholipases, members of which catalyze the hydrolysis of the phospholipid sn-2 ester bond, generating a free fatty acid and a lysophospholipid. PLA2 enzymes are divided into 14 groups (I-XIV), including 20 subgroups (e.g. IA, IB, VIA-1, VIA-2, VIB, etc.), based on sequence homology. Historically, PLA2 enzymes have also been classified based on their biological characteristics into three types, secretory (sPLA2), cytosolic Ca2+-dependent (cPLA2), and cytosolic Ca2+-independent (iPLA2), when sequence data were not available. (PMID 12401193 & 12694560). Cat. No. 07-169-I, Anti-iPLA2, is a purified rabbit polyclonal raised against immunogen sequence present in the group VI SH-iPLA2 & LH-iPLA2 (85/88 kDa calcium-independent phospholipase A2) isoforms, but not found in the 53.2 kDa Ankyrin-iPLA2-1 and 46.5 kDa Ankyrin-iPLA2-2 splice variants encoded by the same PLA2G6 (PLPLA9) gene (UniProt O60733). Immunogen sequence is 100% conserved in murine (NP_058611.1) and rodent (AAC53136.1) species.

免疫原

Epitope: near C-terminal

KLH-conjugated linear peptide corresponding to human iPLA2 near the C-terminal.

應用

Anti-iPLA2 Antibody is an antibody against iPLA2 for use inWestern Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected iPLA2 in human tonsil tissue.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

品質

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected iPLA2 in 10 µg of human testis tissue lysate.

標靶描述

~70 kDa observed (Ramanadham, S., et al. (2003). Biochemistry. 42(47):13929-13940). This antibody is expected to cross-react to isoforms LH-iPLA2 and SH-iPLA2 only.

外觀

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable