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Merck
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主要文件

07-1590

Sigma-Aldrich

Anti-phospho Histone H2A.X Antibody (Tyr142)

from rabbit, purified by affinity chromatography

别名:

Histone H2A.x, H2a/x

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human, mouse

技術

dot blot: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

基因資訊

human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)

一般說明

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The phosphorylated form of H2A.X is thought to be invloved with DNA repair, possibly through reorganizing chromatin into a more open state and allowing the recruitment of proteins to repair DNA double stranded breaks.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human phospho Histone H2A.X phosphorylated at Tyr142.

應用

Anti-phospho Histone H2A.X Antibody (Tyr142) is a highly specific rabbit polyclonal antibody, that targets Histone H2A.X & has been tested in western blotting & Dot Blot.
Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H2A.X (Tyr142) peptides, but not phospho Histone H2A.X (Tyr142) peptides or other peptides corresponding to modified histones.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

品質

Evaluated by Western Blotting in colcemid treated HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2A.X (Tyr142) in colcemid treated HeLa cell lysate.

標靶描述

~16 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外觀

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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