06-284-AF488

Anti-Nitrotyrosine Antibody Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• 别名: Nitrotyrosine AlexaFluor^™ 488 Conjugate
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 物種活性（以同源性預測）: all
• 技術: immunocytochemistry: suitable
• 同型: IgG
• 運輸包裝: wet ice
• 目標翻譯後修改: nitration (Tyr)
• 基因資訊: human ... NOS1(4842)

说明

一般說明

Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.

特異性

This rabbit polyclonal antibody detects tyrosine nitrated proteins.

免疫原

Epitope: Nitrated Tyrosine

Nitrated Keyhole Limpet hemocyanain (KLH).

應用

Anti-Nitrotyrosine AlexaFluor 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.

Research Category
Signaling

Research Sub Category
Developmental Signaling

The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.

品質

Evaluated by Immunocytochemistry in A431 cells treated with peroxytnitrite.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.

外觀

Affinity purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable