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Key Documents

WHA10401116

Whatman® Protran®硝酸纤维素膜

Circles - BA85, pore size 0.45 μm, diam. 82 mm

别名:

Z613673

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About This Item

分類程式碼代碼:
41105339
NACRES:
NB.22

包裝

pkg of 50 ea

製造商/商標名

Whatman 10401116
Whatman Article No. 28414963 (US reference)

直徑

82 mm

孔徑

0.45 μm pore size

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一般說明

Manufactured using 100% pure nitrocellulose to ensure the highest binding capacity. The surface properties of the membrane ensure superior signal-to-noise ratios without the need for stringent washing conditions. The proprietary Protran nitrocellulose formula is proven to provide excellent shelf life of proteins. Evidence shows proteins maintain molecular recognition activity for five years on Protran.

  • Binding capacity, 75 - 110 μg/cm2
  • High binding, low background
  • Triton free matrix
  • Universal membrane for blotting - isotopic, chemluminescent, colorimetric and fluorescent detection methods

Protran nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as ‘nitrocellulose′ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes.

They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric and fluorescent. Unlike PVDF membranes, Protran nitrocellulose does not require a methanol pre-wetting step. This makes Protran the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pre-treatment steps are necessary.

High Binding, Low Background:
In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.

High Retention of Small Proteins:

The 0.2 μm pore size of the Protran (BA83) nitrocellulose membrane has high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through′. The 0.45 μm pore size membrane (BA85) is ideal for larger molecular weight samples. BA79, with 0.1 μm pore size, is the membrane of choice for smaller proteins below 7 kD. A unique benefit of the proprietary Protran nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran.

Protran Blotting Sandwiches:

A pre-cut nitrocellulose membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time. This is made with our Protran BA83 or BA85, the highest quality NC membranes available for blotting.

其他說明

Field of Use : Any microbiological or biotechnological application, including, but not limited to application of membranes for diagnostic, medical, biological, pharmaceutical, consumer product and food and beverage purposes.

法律資訊

Nitrocellulose Membranes (FFHP, Immunopore and BA membranes)
Sold under license to DE10102744 and foreign equivalents thereof
Protran is a registered trademark of Cytiva
Whatman is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

防範說明

危險分類

Flam. Sol. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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