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描述
0.2 μm sterile filtered, suitable for 3D bioprinting applications
形狀
viscous liquid
雜質
≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
顏色
colorless to pale yellow
pH值
6.5-7.5
應用
3D bioprinting
儲存溫度
2-8°C
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一般說明
3D bioprinting is the printing of biocompatible materials, cells, growth factors and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.
應用
Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation. In addition to fast gelation, the methacrylamide functional group can also be used to control the hydrogel physical parameters such as pore size, degradation rate, and swell ratio. Temporal and spatial control of the crosslinking reaction can be obtained by adjusting the degree of functionalization and polymerization conditions, allowing for the fabrication of hydrogels with unique patterns, 3D structures, and morphologies. Gelatin methacrylate based bioinks have been used to bioprint osteogenic, chondrogenic, hepatic, adipogenic, vasculogenic, epithelial, endothelial, cardiac valve, skin, tumor and other tissues and constructs.
包裝
Product contains 10ml of solution packaged in glass bottle.
其他說明
Important tips for optimal bioprinting results
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelMA-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printing cartridge to 15–19 °C to induce gelation, using a temperature controlled printhead or place the cartridge in at 4 °C for 10–15 minutes. If print bed temperature control is available, set temperature to 15 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature.
4. Crosslink: Place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
- Optimize printing conditions (e.g., nozzle diameter, printing speed, printing pressure, temperature, cell density) for the features of your 3D printer and your application.
- Reduce bubble formation. Air bubbles in bioink may hamper bioprinting. Carefully handle the bioink when you mix and transfer it to avoid bubble formation. Do not vortex or shake vigorously.
- UV light Crosslinking. Position the light source directly above the printed structure. Lower intensity light sources will require shorter distances and longer exposure times to complete crosslinking. Recommended conditions: Place an 800 mW/cm2 light source 8 cm above the printed structure and expose for 30 s.
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelMA-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printing cartridge to 15–19 °C to induce gelation, using a temperature controlled printhead or place the cartridge in at 4 °C for 10–15 minutes. If print bed temperature control is available, set temperature to 15 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature.
4. Crosslink: Place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
法律資訊
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
其他客户在看
Jun Yin et al.
ACS applied materials & interfaces, 10(8), 6849-6857 (2018-02-07)
Methacrylated gelatin (GelMA) has been widely used as a tissue-engineered scaffold material, but only low-concentration GelMA hydrogels were found to be promising cell-laden bioinks with excellent cell viability. In this work, we reported a strategy for precise deposition of 5%
Christine McBeth et al.
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Due to its relatively low level of antigenicity and high durability, titanium has successfully been used as the major material for biological implants. However, because the typical interface between titanium and tissue precludes adequate transmission of load into the surrounding
Weitao Jia et al.
Biomaterials, 106, 58-68 (2016-08-24)
Despite the significant technological advancement in tissue engineering, challenges still exist towards the development of complex and fully functional tissue constructs that mimic their natural counterparts. To address these challenges, bioprinting has emerged as an enabling technology to create highly
Marco Costantini et al.
Biofabrication, 8(3), 035002-035002 (2016-07-20)
In this work we demonstrate how to print 3D biomimetic hydrogel scaffolds for cartilage tissue engineering with high cell density (>10(7) cells ml(-1)), high cell viability (85 ÷ 90%) and high printing resolution (≈100 μm) through a two coaxial-needles system.
Thomas Billiet et al.
Biomaterials, 35(1), 49-62 (2013-10-12)
In the present study, we report on the combined efforts of material chemistry, engineering and biology as a systemic approach for the fabrication of high viability 3D printed macroporous gelatin methacrylamide constructs. First, we propose the use and optimization of
商品
Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.
Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.
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