跳转至内容
Merck

861049

Sigma-Aldrich

天青 A 氯化物

certified by the Biological Stain Commission, Dye content 70 %

别名:

Methylene blue

登录查看公司和协议定价


About This Item

经验公式(希尔记法):
C14H14ClN3S
CAS号:
分子量:
291.80
顏色索引編號:
52005
Beilstein:
3922287
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

等級

certified by the Biological Stain Commission

品質等級

形狀

powder

成份

Dye content, 70%

顏色

blue to very dark blue
very dark green

mp

290 °C (dec.) (lit.)

溶解度

H2O: 1 mg/mL

λmax

633 nm

&epsilon ;(消光係數)

≥11000 at 242-246 nm in H2O at -1
≥26000 at 287-291 nm in H2O
≥34000 at 620-634 μm in H2O

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI 密鑰

NALREUIWICQLPS-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

應用

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

生化/生理作用

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

適合性

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Heterochromatin in human male leukocytes.
Lima-de-Faria A and Reitalu J
The Journal of Cell Biology, 16.2, 315-322 (1963)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
Paula Zaman et al.
International journal of nanomedicine, 11, 6149-6159 (2016-12-07)
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors
C H van de Lest et al.
Analytical biochemistry, 221(2), 356-361 (1994-09-01)
A rapid, sensitive, and nonradioactive method has been developed for the quantification and characterization of glycosaminoglycans. The method is based on the separation of different types of glycosaminoglycans in agarose gel and subsequent fixation and staining with the cationic dye

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门