F8146

Anti-Factor VII antibody, Mouse monoclonal

clone HVII-1, purified from hybridoma cell culture

• MDL number: MFCD00164643
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HVII-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): indirect ELISA: suitable; western blot: 1:4,000
• isotype: IgG1
• UniProt accession no.: P08709
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... F7(2155)

Description

General description

Anti-Factor VII antibody, mouse monoclonal (mouse) IgG1 isotype) is derived from the HVII-1 hybridoma produced by the fusion of mouse Sp2/0-Ag14 myeloma cells and splenocytes from immunized BAB/c mice. Human coagulation factor VII contains 10 gamma-carboxyglutamic acid residues (Gla), located at the N-terminal region of the molecule. The activated factor VII (factor VIIa) also consists of a light chain with the Gla-domain and an epidermal growth factor domain, which is linked by a disulfide bond to a heavy chain, containing the serine protease catalytic domain.

Specificity

The antibody recognizes an epitope on the light chain of Factor VII in the presence or absence of divalent cations. Clone no. HVII-1 (also cited as clone no. MC1476/E.A.8.1) may be used for purification of Factor VII and for the preparation of Factor VII depleted human plasma.

Immunogen

Factor VII from pooled normal human plasma.

Application

Anti-Factor VII antibody, Mouse monoclonal has been used in:

• the preparation of factor VII - depleted plasma and for purification
• sandwich-type immunoassays
• the evaluation of patients with hereditary factor VII deficiency by an enzyme linked immunosorbent assay (ELISA) or radio immunosorbent assay (RIA)
• the assay of factor VII level in patients with liver disease (a sensitive parameter of liver dysfunction)
• epidemiological studies of the importance of factor VII level as a risk factor for coronary heart disease, cerebrovascular disease and peripheral vascular diseases
• the determination of the activity state of factor VII in in vivo samples when used with a clotting assay
• flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Monoclonal Anti-Factor VII antibody is suitable for flow cytometry analysis to study the behavior of TF-fVIIa secreted by ovarian cancer cells was associated with microparticles (MPs). It is also suitable for western blot at a dilution of 1:4,000 and indirect ELISA.

Biochem/physiol Actions

Factor VII is a single-chain vitamin K-dependent glycoprotein in human coagulation. It has a molecular weight of ∼50,000Da, present in plasma in trace quantities. It is predominantly synthesized in liver and depends on the posttranslational γ−carboxylation of specific glutamic residues located near the NH2-terminus of the molecule. It is a serine protease zymogen and for activation it requires proteolytic cleavage in the disulfide-linked, two-chain form, Factor VII_a, by Factor X_a, Factor IX_a, thrombin and Factor XII_a or β XII_a.

Physical form

Solution in 10 mM HEPES buffer, pH 7.4, with 140 mM sodium chloride and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable