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MAIPN4550

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, non-sterile

Synonym(s):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate, PVDF Membrane Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

400

description

Non-sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for Avidin-biotin Linkages, DNA-binding Proteins, Protein Binding & PAMPA. Comes in a pack of 50.

sterility

non-sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

parallel artificial membrane permeation assay (PAMPA): suitable

filtration area

0.3 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

shipped in

ambient

Application

MultiScreen®-IP Filter Plate, 0.45 μm, clear, non-sterile has been used:
  • as artificial membrane support and a receiver plate in parallel artificial membrane permeation assays (PAMPA) to determine the permeabilities of oximes
  • in enzyme-linked immunospot (ELISpot) assay
  • in keyhole limpet hemocyanin (KLH)-specific ELISPOT for the identification of antigen (Ag)-specific cells

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Hee Chun Jeong et al.
Bioorganic & medicinal chemistry, 17(17), 6213-6217 (2009-08-12)
A series of fluorinated oxime compounds was designed and synthesized in order to probe the effect of fluorine substitution on reactivation of inhibited acetylcholinesterase (AChE) by organophosphorus agents. Permeability measurements, using the Parallel Artificial Membrane Permeation Assays (PAMPA) method, were

Articles

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

Combining Permeability and Solubility Analyses in a Single Workflow

This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to the donor compartment of the MultiScreen filter plate for the PAMPA analysis.

Protocols

HDM-PAMPA with the MultiScreen Permeability Filter Plate

Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller and Kansy such assays provide rapid, low cost and automation friendly methods to measure a compound’s passive permeability.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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