GE17-1166-01

HiPrep^™ 16/60 Sephacryl^® S-200 HR

Cytiva 17-1166-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

• Synonym(s): Sephacryl S-200 column
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: polypropylene
• form: prepacked column
• crosslinking: cross-linked
• mol wt: 5000-250000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 17-1166-01
• storage condition: (20% Ethanol)
• parameter: <1 mL/min flow rate; <5 mL sample volume; 0.15 Mpa (22 psi); 22 psi
• technique(s): liquid chromatography (LC): suitable
• bed volume: 120 mL
• column L × I.D.: 60 cm × 16 mm
• matrix: dextran-acrylamide particle platform; allyl dextran and N, N’-methylenebisacrylamide; polymeric
• matrix active group: dextran-acrylamide phase
• avg. part. size: 50 μm
• cleaning: 2-13
• working range: 3-11
• separation technique: size exclusion (SEC)

Description

General description

HiPrep^™ 16/60 Sephacryl^® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl^® S-200 is used for separating midsize proteins including antibodies and serum proteins.

Features and Benefits

• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
• Gel filtration columns for low to medium pressure AKTA systems.
• Well-established in industrial production.
• The HiPrep^™ Sephacryl^® series offers reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep^™ Sephacryl^® columns speed up protein purification.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

Gel filtration columns for low to medium pressure ÄKTA systems.

for preparative separation of biomolecules of up to 5 mL sample volume

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids