S300HR

Sephacryl^®

300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)

• Synonym(s): Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
• CAS Number: 65546-95-4
• MDL number: MFCD00165824
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• Quality Level: 200
• description: stability: stable to 0.2M NaOH
• form: suspension (20% aqueous ethanol)
• feature: autoclavable
• matrix: crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
• particle size: 25-75 μm (wet)
• pore size: 1-400 kDa MW range (dextrans); 10-1500 kDa MW range (globular proteins)
• compatibility: mode of use size exclusion chromatography
• storage temp.: 2-8°C

Description

General description

S300HR-750ML′s updated product number is GE17-0599-01

• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)

Application

Sephacryl^® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl^® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl^® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 100.4 - 109.4 °F - closed cup
• Flash Point(C): 38 - 43 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves