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Merck
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Principaux documents

Y1771

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

Synonyme(s) :

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements

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About This Item

Numéro MDL:
MFCD01095679
Code UNSPSC :
41106212
Nomenclature NACRES :
NA.85

Niveau de qualité

200

Forme

powder

Technique(s)

transformation: suitable

Application(s)

microbiology

Température de stockage

room temp

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Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

  • the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
  • Yarrowia lipolytica PO1f strain positive transformants
  • CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Notes préparatoires

Dissolve 1.46 g of supplement per liter of medium.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

G7021

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

Gélose, for molecular biology

Y1501

Yeast Synthetic Drop-out Medium Supplements, without uracil

Y2001

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil

Y0626

Base azotée pour levures sans acides aminés, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1896

Yeast Synthetic Drop-out Medium Supplements, without lysine

Y2021

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine

Y2146

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H318

Conseils de prudence

P280 - P305 + P351 + P338

Classification des risques

Eye Dam. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
099M4134V
11190505
029M4027V
018M4087V
11171022

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps

Articles

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocoles

Yeast Drop Out

Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Contenu apparenté

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced...

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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