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YEAST1

Sigma-Aldrich

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

Synonyme(s) :

lithium acetate yeast transformation

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.85

Qualité

for molecular biology

Niveau de qualité

200

Utilisation

 kit sufficient for >100 standard transformations

Technique(s)

transformation: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Caractéristiques et avantages

  • Easy and ready-to-use
  • Requires as little as 10 ng of plasmid DNA
  • Flexibility for any strain of yeast
  • Sufficient for over 100 standard transformations

Composants

The Yeast Transformation Kit contains:
  • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
  • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
  • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
  • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
  • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Principe

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1FDS
  • Y1501Yeast Synthetic Drop-out Medium Supplements, without uracil 1 gFDS

Produit(s) apparenté(s)

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Tarif

D8418

Diméthylsulfoxyde, for molecular biology

A5306

Gélose, for molecular biology

G5516

Glycérol, for molecular biology, ≥99.0%

Y1625

Extrait de levure, for use in microbial growth medium

Y0750

Yeast Synthetic Drop-out Medium Supplements, without leucine and tryptophan

Y1751

Yeast Synthetic Drop-out Medium Supplements, without histidine

Y2001

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil

Y1376

Yeast Synthetic Drop-out Medium Supplements, without leucine

Y0626

Base azotée pour levures sans acides aminés, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1876

Yeast Synthetic Drop-out Medium Supplements, without tryptophan

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Mechanistic and structural insights into the regioselectivity of an acyl-CoA fatty acid desaturase via directed molecular evolution.
Vanhercke T
The Journal of Biological Chemistry, 286(15), 12860-12869 (2011)
Widening the pH activity profile of a fungal laccase by directed evolution.
Torres-Salas P
Chembiochem, 14(8), 934-937 (2013)
A simple and efficient procedure for transformation of yeasts.
R Elble
BioTechniques, 13(1), 18-20 (1992-07-01)
Afficher tous les articles scientifiques apparentés

Articles

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protocoles

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

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