V7259

Vascular Endothelial Growth Factor human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonyme(s) : VEGF
• Numéro CAS: 127464-60-2
• Numéro MDL: MFCD00286542
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Vascular Endothelial Growth Factor human, VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (SDS-PAGE)
• Forme: powder
• Puissance: 1-10 ng/mL ED₅₀/EC₅₀
• Qualité: endotoxin tested
• Poids mol.: protein 38.2 kDa (as homodimer)
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤0.1 EU/μg
• Couleur: white
• Solubilité: water: soluble
• Numéro d'accès UniProt: P15692
• Température de stockage: −20°C
• Informations sur le gène: human ... VEGFA(7422)

Description

Description générale

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

Application

Vascular Endothelial Growth Factor (VEGF) has been used for-

• the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
• inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Actions biochimiques/physiologiques

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Propriétés physiques

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Forme physique

Lyophilized from a sterile filtered buffered solution with no additives.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)