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V7259

Sigma-Aldrich

Vascular Endothelial Growth Factor human

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonyme(s) :

VEGF

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About This Item

Numéro CAS:
127464-60-2
Numéro MDL:
MFCD00286542
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

≥98% (SDS-PAGE)

Forme

powder

Puissance

1-10 ng/mL ED50/EC50

Qualité

endotoxin tested

Poids mol.

protein 38.2 kDa (as homodimer)

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤0.1 EU/μg

Couleur

white

Solubilité

water: soluble

Numéro d'accès UniProt

P15692

Température de stockage

−20°C

Informations sur le gène

human ... VEGFA(7422)

Catégories apparentées

Description générale

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

Application

Vascular Endothelial Growth Factor (VEGF) has been used for-
  • the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
  • inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Actions biochimiques/physiologiques

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Propriétés physiques

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Forme physique

Lyophilized from a sterile filtered buffered solution with no additives.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Behrous Davani et al.
Stem cells (Dayton, Ohio), 25(12), 3215-3222 (2007-09-29)
Islet transplantation offers improved glucose homeostasis in diabetic patients, but transplantation of islets is limited by the supply of donor pancreases. Undifferentiated precursors hold promise for cell therapy because they can expand before differentiation to produce a large supply of
Chih-Hung Lai et al.
Frontiers in cell and developmental biology, 9, 635307-635307 (2021-03-02)
The homeostasis of vascular endothelium is crucial for cardiovascular health and endothelial cell (EC) aging and dysfunction could negatively impact vascular function. Leveraging transcriptome profiles from ECs subjected to various stimuli, including time-series data obtained from ECs under physiological pulsatile
Kazuhide Hayakawa et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(29), 10666-10670 (2011-07-22)
Originally identified as an angiogenic factor, vascular endothelial growth factor (VEGF-A) is now known to play multiple roles in the CNS, including the direct regulation of neuronal and astrocytic functions. Here, we ask whether VEGF-A can also have a novel
Michael Bressan et al.
Development (Cambridge, England), 141(21), 4149-4157 (2014-10-03)
Efficient blood flow depends on two developmental processes that occur within the atrioventricular junction (AVJ) of the heart: conduction delay, which entrains sequential chamber contraction; and valve formation, which prevents retrograde fluid movement. Defects in either result in severe congenital
The biology of vascular endothelial growth factor.
N Ferrara et al.
Endocrine reviews, 18(1), 4-25 (1997-02-01)
Afficher tous les articles scientifiques apparentés

Articles

Vascular Endothelial Growth Factors (VEGF)

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell migration, proliferation, tube formation, and survival. In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization.

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