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R9894

Sigma-Aldrich

Ras human

Wild type, recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonyme(s) :

H-Ras, rH-RAS, WT

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About This Item

Numéro MDL:
MFCD00241858
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Produit recombinant

expressed in E. coli

Pureté

≥80% (SDS-PAGE)

Forme

buffered aqueous glycerol solution

Poids mol.

28 kDa

Numéro d'accès UniProt

Q0VAM2

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... RASGEF1B(153020)

Description générale

Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.

Application

Human Ras has been used as a negative control in tubulin overlay assay.

Actions biochimiques/physiologiques

Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.

Conditionnement

Package size based on protein content

Forme physique

Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
Jean-Luc D
The Journal of Biological Chemistry (1997)
Slow conformational dynamics of the guanine nucleotide-binding protein Ras complexed with the GTP analogue GTPgammaS.
Spoerner M
FEBS Journal (2007)
J Tucker et al.
The EMBO journal, 5(6), 1351-1358 (1986-06-01)
v-Ha-ras encoded p21 protein (p21V), the cellular c-Ha-ras encoded protein (p21C) and its T24 mutant form p21T were produced in Escherichia coli under the control of the tac promoter. Large amounts of the authentic proteins in a soluble form can
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Test (2006)
Structure and function of p21 ras proteins
Shih TY, et al.
Gene Analysis Techniques (1986)
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