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Principaux documents

SRP3136

Sigma-Aldrich

PAF-AH human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonyme(s) :

1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, LDL-associated phospholipase A2, PAF 2-acylhydrolase, PAF acetylhydrolase

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in HEK 293 cells

Essai

≥95% (HPLC)
≥95% (SDS-PAGE)

Forme

lyophilized

Puissance

≤0.1 ng/mL ED50

Poids mol.

47-55 kDa

Conditionnement

pkg of 20 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white

Numéro d'accès UniProt

Q13093

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... PLA2G7(7941)

Description générale

Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55kDa by SDS-PAGE analysis.

Actions biochimiques/physiologiques

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet activating factor acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL (low density lipoprotein) particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. High levels of PAF-AH in plasma can be an indication of resistant hypertension. Polymorphism in PAF-AH gene at A379V might be linked with ischemic stroke susceptibility.

Séquence

FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN

Forme physique

Lyophilized from 10 mM Tris, pH 8.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0416448
1008448
0608448

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Consulter la Bibliothèque de documents

Hiroyuki Arai
Prostaglandins & other lipid mediators, 68-69, 83-94 (2002-11-16)
Platelet-activating factor (PAF) is one of the most potent lipid mediators and is involved in a variety of physiological events. The acetyl group at the sn-2 position of its glycerol backbone is required for its biological activity, and deacetylation of
Biology of platelet-activating factor acetylhydrolase (PAF-AH, lipoprotein associated phospholipase A2).
Stafforini DM
Cardiovascular Drugs and Therapy, 23, 73-83 (2009)
Platelet-activating factor induces NF-kappa B activation through a G protein-coupled pathway.
Kravchenko VV, et al.
The Journal of Biological Chemistry, 270, 14928-14934 (1995)
Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung.
Honda Z, et al.
Nature, 349, 342-346 (1991)
Association of PLA2G7 gene polymorphisms with ischemic stroke in northern Chinese Han population.
Liu X, et al.
Clinical Biochemistry, 47, 404-408 (2014)
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