SRP0291
Cathepsin L Active human
recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)
Synonyme(s) :
CATL, Major excreted protein (MEP)
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Produit recombinant
expressed in FreeStyle™ 293-F cells
Pureté
≥90% (SDS-PAGE)
Forme
aqueous solution
Poids mol.
36 kDa
Numéro d'accès NCBI
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... CTSL(1514)
Description générale
Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.
Application
Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used in a study to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases. Active human cathepsin L has also been used in a study to investigate the role of cathepsin B and L activity in the serum during the human aging process.
Définition de l'unité
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
Forme physique
Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.
Notes préparatoires
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Informations légales
FreeStyle is a trademark of Invitrogen Corp.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ricardo A Orbegozo-Medina et al.
PloS one, 14(2), e0211035-e0211035 (2019-02-02)
Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host
Jana Ilgová et al.
Molecular and biochemical parasitology, 235, 111248-111248 (2019-12-25)
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably
Elda A Treviño et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(2), 490-502 (2018-11-21)
The etiology of joint tissue degeneration following rotator cuff tear remains unclear. Thus, the purpose of this study was to understand the timeline of protease activity in the soft tissues of the shoulder (tendon, muscle, and cartilage) that may lead
Aleksandra Wyczałkowska-Tomasik et al.
Archives of gerontology and geriatrics, 55(3), 735-738 (2012-06-15)
Cathepsin B and L are active in several physiological processes that occur in the human body. Their synthesis and activity is detectable in several tissues and processes, including skin hyperkeratosis, insemination, and embryonic development. Proteolytic enzymes are very important cellular
Ana Bela Santana et al.
Bioorganic & medicinal chemistry letters, 22(12), 3993-3997 (2012-05-19)
The synthesis, inhibitory activity and mode of action of oxazolidine-2,4-diones against porcine pancreatic elastase, here used as a model for human neutrophil elastase, are reported. The nature of N-substitution at the oxazolidine-2,4-dione scaffold has large effect on the inhibitory potency
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique