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SAB4200724

Sigma-Aldrich

Anti-AGO2 antibody, Mouse monoclonal

clone AGO2-10, purified from hybridoma cell culture

Synonyme(s) :

Anti-Argonaute RISC catalytic component 2, Anti-Argonaute2, Anti-Eukaryotic translation initiation factor 2C 2, Anti-PAZ Piwi domain protein (PPD), Anti-Protein Argonaute 2, Anti-eIF2C 2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

AGO2-10, monoclonal

Forme

buffered aqueous solution

Poids mol.

~95 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

immunoblotting: 2-4 μg/mL using extract of HEK-293T cells transfected with human AGO2
immunoprecipitation (IP): 10-20 μg/test using HeLa cells extract

Isotype

IgG1

Numéro d'accès UniProt

Q9UKV8

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... AGO2(27161)

Description générale

Anti-AGO2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the AGO2-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide from the C-terminal region of human AGO2 protein, conjugated to keyhole limpet hemocyanin (KLH). AGO2 is also known as protein argonaute 2, argonaute RNA-induced silencing complex (RISC) catalytic component 2, eukaryotic translation initiation factor 2C 2 (eIF2C2) and PAZ Piwi domain protein (PPD). Argonaute 2 (AGO2) belongs to the Argonaute family. It is located on human chromosome 8q24. Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.

Immunogène

synthetic peptide from the C-terminal region of human AGO2 protein, conjugated to KLH

Application

Anti-AGO2 antibody, Mouse monoclonal has been used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

Argonaute 2 (AGO2) exhibits intrinsic endonuclease activity. It can directly cleave mRNAs in a small RNA-guided manner. AGO2 plays a central role in RNA silencing processes as essential catalytic components of the RNA-induced silencing complex (RISC). It has an important role in stabilization of circulating microRNAs (miRNAs) in exosomes from extracellular body fluids.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
096M4786

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Lasse Peters et al.
Molecular cell, 26(5), 611-623 (2007-06-15)
Small regulatory RNAs such as short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi interacting RNAs (piRNAs) have been discovered in the past, and it is becoming more and more apparent that these small molecules have key regulatory functions. Small RNAs
Andrey Turchinovich et al.
Methods in molecular biology (Clifton, N.J.), 1024, 97-107 (2013-05-31)
Circulating microRNAs (miRNAs) have been recently detected in extracellular body fluids and proved themselves as promising biomarkers for a broad spectrum of diseases. The techniques to isolate, detect, and characterize extracellular miRNAs vary significantly from report to report. In this
Jidong Liu et al.
Science (New York, N.Y.), 305(5689), 1437-1441 (2004-07-31)
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) and Argonaute family proteins. Here, we show that the multiple Argonaute proteins present in mammals are
Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma
Cheng N, et al.
Hepatology, 57(5), 1906-1918 (2013)
Shao-Chun Wu et al.
PloS one, 8(10), e77936-e77936 (2013-11-10)
The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was
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