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SAB4200119

Sigma-Aldrich

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat

2-4 mg/mL, clone 6F7, purified immunoglobulin

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32

Source biologique

rat

Niveau de qualité

200

Conjugué

peroxidase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

6F7, monoclonal

Forme

buffered aqueous solution

Espèces réactives

all

Concentration

2-4 mg/mL

Technique(s)

western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein

Isotype

IgG1

Séquence immunogène

DYKDDDDK

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide.
The product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.

Immunogène

purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP).

Application

Learn more product details in our FLAG® applications portal.
Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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DongHyuk Lee et al.
Nature communications, 11(1), 1838-1838 (2020-04-17)
Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive.
Joseph J Boyle et al.
The American journal of pathology, 174(3), 1097-1108 (2009-02-24)
Intraplaque hemorrhage accelerates atherosclerosis via oxidant stress and contributes to lesion development and destabilization. Normally, macrophages scavenge hemoglobin-haptoglobin (HbHp) complexes via CD163, and this process provokes the secretion of the anti-inflammatory atheroprotective cytokine interleukin (IL)-10. We therefore tested the hypothesis
Claudia Isabelle Keller Valsecchi et al.
Nature communications, 9(1), 3626-3626 (2018-09-09)
Haploinsufficiency and aneuploidy are two phenomena, where gene dosage alterations cause severe defects ultimately resulting in developmental failures and disease. One remarkable exception is the X chromosome, where copy number differences between sexes are buffered by dosage compensation systems. In
Elizabeth A Libby et al.
PLoS genetics, 11(6), e1005275-e1005275 (2015-06-24)
Most bacteria contain both eukaryotic-like Ser/Thr kinases (eSTKs) and eukaryotic-like Ser/Thr phosphatases (eSTPs). Their role in bacterial physiology is not currently well understood in large part because the conditions where the eSTKs are active are generally not known. However, all

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