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Merck
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Key Documents

S5394

Sigma-Aldrich

Lipoprotein Deficient Serum from fetal calf

frozen liquid

Synonyme(s) :

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.61

Source biologique

bovine fetus

Niveau de qualité

200

Forme

frozen liquid

Concentration

≥14 mg protein/mL Biuret

Technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Impuretés

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.

Application

Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).

Notes préparatoires

Aseptically filtered, 0.2 μm
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

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Consulter la Bibliothèque de documents

P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Utsab Subedi et al.
International journal of molecular sciences, 24(3) (2023-02-12)
Neointimal hyperplasia is characterized by a loss of the contractile phenotype of vascular smooth muscle cells (VSMCs). Our group has recently shown that VSMC proliferation and migration are mediated by lysophosphatidic acid (LPA) during restenosis, but the role of autotaxin
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
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