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Sigma-Aldrich

PeroxiDetect™ Kit

Synonyme(s) :

Peroxidase assay kit

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Température de stockage

2-8°C

Description générale

The PeroxiDetect Kit For the Determination of Aqueous and Lipid Hydroperoxides was developed for the measurement of peroxides in biological systems, which is an important factor in determining the degree of free radicals present in specific tissues.

Application

For the determination of aqueous peroxides and lipid hydroperoxides. Peroxides serve as a source for hydroxyl or peroxyl reactive radicals. These radicals can interact with DNA, proteins or lipid components of cells, causing cell damage that could lead to cell death. The measurement of peroxides in biological systems is an important factor in determining the degree of free radicals present in specific tissues. Lipid peroxidation has been proposed to contribute to various pathophysiological cell and tissue abnormalities. For example, increased levels of lipid peroxidation products in red blood cells were found to correlate well with the onset of diabetes mellitus in pregnant women. Cholesterol hydroperoxides accumulate in patients having excessive blood alcohol. Measurement of hydrogen peroxide in tissues has been used to study several aspects of free radical damage such as skin aging as induced by UV light and the effect of H2O2 as an inducer of elevated tyrosinase levels in melanoma cells. H2O2 has been shown to be a potent mitogen for growth-arrested cultured human aortic smooth muscle cells. The PeroxiDetect kit is capable of detection of H2O2 in aqueous solutions in the range of 1-7 nanomoles per reaction volume or of lipid hydroperoxides in organic solvents in the range of 1-16 nanomoles per reaction volume. This kit is based on the fact that peroxides will convert Fe2+ ion to Fe3+ ion at acidic pH. The Fe3+ ion will form a colored adduct with xylenol orange which is observed at 560 nm.
PeroxiDetect™ Kit is suitable for use:
  • in the quantification of hydroperoxides in synaptosomal preparation
  • in measuring lipid peroxidation in melanoma cells
  • to quantify peroxides in the cerebral cortex
  • intestinal lumen sample

Peroxides will convert Fe2+ to Fe3+ ions under acidic conditions. Fe3+ ions will then form a colored adduct with xylenol orange, which is observed at 560 nm.

Composants

Kit contains reagents for 100 tests of aqueous peroxide and 100 tests of organic peroxide.

Informations légales

PeroxiDetect is a trademark of Sigma-Aldrich Co. LLC

Composants de kit seuls

Réf. du produit
Description
  • tert-Butyl hydroperoxide 1 mL
  • Hydrogen peroxide 1 mL

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Org. Perox. C - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

5.2 - Organic peroxides and self-reacting hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Ana Sofia Azevedo et al.
BMC developmental biology, 12, 24-24 (2012-08-28)
Zebrafish has emerged as a powerful model organism to study the process of regeneration. This teleost fish has the ability to regenerate various tissues and organs like the heart, spinal cord, retina and fins. In this study, we took advantage
Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
Dinvcic M, et al.
International Journal of Radiation Biology, 94(11), 1062-1071 (2018)
Effects of Humulus lupulus extract or its Components on Viability, Lipid Peroxidation, and expression of Vascular Endothelial Growth Factor in Melanoma Cells and Fibroblasts
Philips N, et al.
Madridge Journal of Clinical Research, 1, 15-19 (2017)
Altered muscarinic receptor expression in the cerebral cortex of epileptic rats: restorative role of Withania somnifera
Anju TR, et al.
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire, 96(4), 433-440 (2017)
J M Herbert et al.
FEBS letters, 395(1), 43-47 (1996-10-14)
Increased generation of active oxygen species such as hydrogen peroxide (H202) may be important in vascular smooth muscle cell growth associated with atherosclerosis and restenosis. In this work, we showed that H202 was a potent mitogen for growth-arrested cultured human
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