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P7306

Sigma-Aldrich

Polyethylene glycol/ dimethyl sulfoxide solution

Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

Synonyme(s) :

PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO

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About This Item

Numéro MDL:
MFCD00081839
Code UNSPSC :
12161902
ID de substance PubChem :
24898839
Nomenclature NACRES :
NA.75

Qualité

Hybri-Max™

Niveau de qualité

200

Stérilité

0.2 μm filtered

Gamme de produits

BioReagent

Forme

solution

Poids mol.

average mol wt 1,450

Concentration

50 % (w/v)

Technique(s)

cell culture | hybridoma: suitable

Impuretés

endotoxin, tested

Température de stockage

2-8°C

Chaîne SMILES 

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

Clé InChI

LYCAIKOWRPUZTN-UHFFFAOYSA-N

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Conditionnement

Packaged in sealed ampules under nitrogen.

Autres remarques

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Wen-Hung Chen et al.
Virology journal, 14(1), 189-189 (2017-10-04)
Dengue virus (DV) infection causes a spectrum of clinical diseases ranging from dengue fever to a life-threatening dengue hemorrhagic fever. Four distinct serotypes (DV1-4), which have similar genome sequences and envelope protein (E protein) antigenic properties, were divided. Among these
Wei Guo et al.
Neurobiology of disease, 132, 104590-104590 (2019-08-31)
While Brain-derived Neurotrophic Factor (BDNF) has long been implicated in treating neurological diseases, recombinant BDNF protein has failed in multiple clinical trials. In addition to its unstable and adhesive nature, BDNF can activate p75NTR, a receptor mediating cellular functions opposite
Indre Kucinskaite-Kodze et al.
Biomolecules, 10(7) (2020-07-12)
The pathogenicity of many bacteria, including Streptococcus pneumoniae, depends on pore-forming toxins (PFTs) that cause host cell lysis by forming large pores in cholesterol-containing cell membranes. Therefore, PFTs-neutralising antibodies may provide useful tools for reducing S. pneumoniae pathogenic effects. This
Liliana R Loureiro et al.
Scientific reports, 8(1), 12196-12196 (2018-08-17)
Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen. Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities
Sondra T Bland et al.
Brain, behavior, and immunity, 23(4), 492-497 (2009-06-03)
Glial activation has recently been discovered to modulate several effects of morphine, including analgesia, tolerance, and dependence. The present studies extend this line of investigation by exploring whether glial activation may also affect extracellular levels of dopamine (DA) in the
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