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H0137

Sigma-Aldrich

HT Media Supplement (50×) Hybri-Max™

lyophilized powder, suitable for hybridoma, BioXtra

Synonyme(s) :

HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

HT Media Supplement (50×) Hybri-Max™, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

Niveau de qualité

200

Stérilité

γ-irradiated

Gamme de produits

BioXtra

Forme

lyophilized powder

Technique(s)

cell culture | hybridoma: suitable

Impuretés

endotoxin, tested

Température de stockage

−20°C

Application

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™ has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii
Gondim L, et al.
Experimental Parasitology, 163, 46-56 (2016)
Simulated Cholinergic Reinnervation of beta (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing beta Cell and Cholinergic Cell
Jiao A, et al.
International Journal of Endocrinology, 2018 (2018)
The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress
Hashiguchi K, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 808, 10-19 (2018)
Kimberly A McCormack et al.
Parasitology research, 118(8), 2409-2417 (2019-06-15)
Human babesiosis, a tick-borne disease similar to malaria, is most often caused by the hemoprotozoans Babesia divergens in Europe, and Babesia microti and Babesia duncani in North America. Babesia microti is the best documented and causes more cases of human
Emmanuel Osei Mensah et al.
Journal of bioscience and bioengineering, 128(4), 487-494 (2019-04-30)
Since the use of protein therapeutics is effective for treating intractable human diseases, the production of biologic therapeutic agents has dramatically increased over the past three decades. The Chinese hamster ovary (CHO) cell lines are the most commonly used host
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Protocoles

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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