Accéder au contenu
Merck
Toutes les photos(1)

Documents

P5511

Sigma-Aldrich

Protein Kinase A from bovine heart

≥0.8 units/μg protein, lyophilized powder

Synonyme(s) :

Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
9026-43-1
Numéro de classification (Commission des enzymes):
2.7.1.37 (BRENDA, IUBMB)
Numéro MDL:
MFCD00132121
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.77

Forme

lyophilized powder

Activité spécifique

≥0.8 units/μg protein

Composition

Protein, ≥70%

Numéro d'accès UniProt

P00517
P05131

Température de stockage

−20°C

Informations sur le gène

cow ... PRKACA(282322) , PRKACB(282323)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

Application

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

Actions biochimiques/physiologiques

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

Forme physique

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Notes préparatoires

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Remarque sur l'analyse

Protein determined by biuret.

Inhibiteur

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ilse A E Bollen et al.
Frontiers in physiology, 8, 1103-1103 (2018-01-10)
Dilated cardiomyopathy amongst children (pediatric cardiomyopathy, pediatric CM) is associated with a high morbidity and mortality. Because little is known about the pathophysiology of pediatric CM, treatment is largely based on adult heart failure therapy. The reason for high morbidity
Signaling the signal: Protein Kinase a (PKA) inhibition by insulin-formed H2O2 and reactivation by thioredoxin
de Pina MZ, et al.
The Journal of Biological Chemistry (2008)
Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
Journal of Bioenergetics and Biomembranes (2017)
Alkylation of Staurosporine to Derive a Kinase Probe for Fluorescence Applications
Dr. Alexandar
Chemistry of Materials (2016)
Nitroglycerine-and isoprenaline-induced vasodilatation: assessment from the actions of cyclic nucleotides
Itohy T, et al.
British Journal of Pharmacology, 84(2), 393-406 (1985)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique